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Phosphopantetheinyl transferases PPTaseswhich play an essential role in both primary and secondary metabolism, are magnesium binding enzymes. In this study, we characterized the magnesium binding residues of all known group II PPTases by biochemical and evolutionary analysis. Mutations of two three-magnesium-binding-residue-PPTases and one two-magnesium-binding-residue-PPTase indicate that the first and the third residues in the triads are essential to activities; the second residues in the triads are non-essential.

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Some errors that arise are noted below, along with more details on what the issue is and how to solve it. General 1. Cannot allocate memory 2.

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